WB
Western blot analysis of anti-PRTN3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRTN3 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of PRTN3 using anti-PRTN3 antibody. PRTN3 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of Myeloblastin/PRTN3 using anti-Myeloblastin/PRTN3 antibody. Myeloblastin/PRTN3 was detected in an immunocytochemical section of THP1 cells. The section was incubated with rabbit anti-Myeloblastin/PRTN3 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of THP-1 cells using anti-PRTN3 antibody. Overlay histogram showing THP-1 cells stained with Rabbit polyclonal antibody to Myeloblastin/PRTN3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRTN3 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to Myeloblastin/PRTN3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human PRTN3 recombinant protein (Position: A23-D241). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRTN3 |
|---|---|
| Gene Synonyms | MBN MBT NP4 P29 PR3 ACPA AGP7 NP-4 PR-3 CANCA C-ANCA |
| Gene Full Name | proteinase 3 |
| Gene Summary | Enables enzyme binding activity; serine-type endopeptidase activity; and signaling receptor binding activity. Involved in several processes, including mature conventional dendritic cell differentiation; neutrophil extravasation; and positive regulation of GTPase activity. Located in azurophil granule lumen; cytosol; and plasma membrane raft. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | ACPA AGP7 C ANCA C ANCA antigen Leukocyte proteinase 3 MBN MBT Myeloblastin Neutrophil proteinase 4 P29 PR 3 PR3 proteinase 3 PRTN3 Wegener autoantigen |
| Molecular Weight(MW) | 28 kDa |
| Cellular Localization | Cell membrane,Membrane,Secreted. |
WB
Western blot analysis of anti-PRTN3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRTN3 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of PRTN3 using anti-PRTN3 antibody. PRTN3 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of Myeloblastin/PRTN3 using anti-Myeloblastin/PRTN3 antibody. Myeloblastin/PRTN3 was detected in an immunocytochemical section of THP1 cells. The section was incubated with rabbit anti-Myeloblastin/PRTN3 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of THP-1 cells using anti-PRTN3 antibody. Overlay histogram showing THP-1 cells stained with Rabbit polyclonal antibody to Myeloblastin/PRTN3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRTN3 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
