WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.IHC
Human brain was stained with anti-PKR rabbit antibody.IHC
Rat brain was stained with anti-PKR rabbit antibody.ICC/IF
Immunofluorescence analysis of HEK293. Picture A: PKR antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | PKR Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000-1:10000 IHC:1:100-1:500 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EIF2AK2 |
|---|---|
| Gene Synonyms | PKR PRKR PPP1R83 |
| Gene Full Name | eukaryotic translation initiation factor 2 alpha kinase 2 |
| Gene Summary | The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. The encoded protein plays an important role in the innate immune response against multiple DNA and RNA viruses. [provided by RefSeq, Jul 2021] |
| Alternative Names | EIF2AK2 PKR PRKR Interferon-induced double-stranded RNA-activated protein kinase Eukaryotic translation initiation factor 2-alpha kinase 2 eIF-2A protein kinase 2 Interferon-inducible RNA-dependent protein kinase P1/eIF-2A protein k |
| Molecular Weight(MW) | 62 kDa |
| Cellular Localization | Cytoplasm . Nucleus . Cytoplasm , perinuclear region . Nuclear localization is elevated in acute leukemia , myelodysplastic syndrome (MDS) , melanoma , breast , colon , prostate and lung cancer patient samples or cell lines as well as neurocytes from advanced Creutzfeldt-Jakob disease patients. . |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.
IHC
Human brain was stained with anti-PKR rabbit antibody.
IHC
Rat brain was stained with anti-PKR rabbit antibody.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: PKR antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:2000-1:10000 IHC:1:100-1:500 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
