WB
Western blot analysis of lysates from TT cells using CGRP Rabbit mAb at 1:20000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1 s.IHC
Immunohistochemistry analysis of paraffin embedded Human thyroid medullary carcinoma tissue using CGRP Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.ICC/IF
Confocal imaging of TT cells and 293T cells (negative) using CGRP Rabbit mAb ( dilution 1:20000) followed by a further incubation with Cy3-conjugated Goat anti Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488 conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.| Product Name | CGRP Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:10000 - 1:60000 IHC:1:500 - 1:2000 ICC/IF:1:10000 - 1:20000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CALCA |
|---|---|
| Gene Synonyms | CT KC PCT CGRP CALC1 CGRP1 CGRP-I CGRP-alpha |
| Gene Full Name | calcitonin related polypeptide alpha |
| Gene Summary | This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014] |
| Alternative Names | CT KC PCT CGRP CALC1 CGRP1 CGRP-I CGRP-alpha |
| Molecular Weight(MW) | 14 kDa(Observed band size:5 kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of lysates from TT cells using CGRP Rabbit mAb at 1:20000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1 s.
IHC
Immunohistochemistry analysis of paraffin embedded Human thyroid medullary carcinoma tissue using CGRP Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ICC/IF
Confocal imaging of TT cells and 293T cells (negative) using CGRP Rabbit mAb ( dilution 1:20000) followed by a further incubation with Cy3-conjugated Goat anti Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488 conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.| Application Notes | WB:1:10000 - 1:60000 IHC:1:500 - 1:2000 ICC/IF:1:10000 - 1:20000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
