WB
Western blot analysis of ANGPT2 using anti-ANGPT2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ANGPT2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
ANGPT2 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ANGPT2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
ANGPT2 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ANGPT2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ANGPT2 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-ANGPT2 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Overlay histogram showing U937 cells stained withrabbit anti-ANGPT2 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANGPT2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to ANGPT2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human Angiopoietin-2/ANGPT2 recombinant protein (Position: N357-K404). Human ANGPT2 shares 83% amino acid (aa) sequence identity with both mouse and rat ANGPT2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ANGPT2 |
|---|---|
| Gene Synonyms | ANG2 AGPT2 LMPHM10 |
| Gene Full Name | angiopoietin 2 |
| Gene Summary | This gene belongs to the angiopoietin family of growth factors. The protein encoded by this gene is an antagonist of angiopoietin 1, and both angiopoietin 1 and angiopoietin 2 are ligands for the endothelial TEK receptor tyrosine kinase. Angiopoietin 2 is upregulated in multiple inflammatory diseases and is implicated in the direct control of inflammation-related signaling pathways. The encoded protein affects angiogenesis during embryogenesis and tumorigenesis, disrupts the vascular remodeling ability of angiopoietin 1, and may induce endothelial cell apoptosis. This gene serves a prognostic biomarker for acute respiratory distress syndrome. [provided by RefSeq, Aug 2020] |
| Alternative Names | AGPT2 ANG 2 ANG2 angiopoietin 2 Angiopoietin-2 ANGPT2 PDGFAB |
| Molecular Weight(MW) | 51 kDa(Observed band size:70 kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of ANGPT2 using anti-ANGPT2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ANGPT2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
ANGPT2 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ANGPT2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
ANGPT2 was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ANGPT2 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ANGPT2 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-ANGPT2 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Overlay histogram showing U937 cells stained withrabbit anti-ANGPT2 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ANGPT2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
