WB
Western blot analysis of FAM38A / PIEZO1 on different lysates with Rabbit anti-FAM38A / PIEZO1 antibody at 1/2,000 dilution. Lane 1: 293T transfected with mPiezo1 cell lysate, Lane 2: 293T transfected with mPiezo2 cell lysate (negative), Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of 293T cells labeling FAM38A / PIEZO1 with Rabbit anti-FAM38A / PIEZO1 antibody at 1/200 dilution. 293T cells, transfected with GFP-tagged mouse Piezo1 (top, positive) / tag-free mouse Piezo2 (bottom, negative) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FAM38A / PIEZO1 antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.| Product Name | FAM38A / PIEZO1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse PIEZO1 aa 2,214-2,457. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:2000 ICC/IF:1:200 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Piezo1 |
|---|---|
| Gene Synonyms | Fam38a mKIAA0233 9630020g22 |
| Gene Full Name | piezo-type mechanosensitive ion channel component 1 |
| Gene Summary | Enables identical protein binding activity and mechanosensitive monoatomic cation channel activity. Involved in monoatomic cation transport and positive regulation of myotube differentiation. Acts upstream of or within detection of mechanical stimulus and regulation of membrane potential. Located in cuticular plate; plasma membrane; and stereocilium. Is expressed in several structures, including brain; cardiovascular system; hemolymphoid system; liver; and lung. Human ortholog(s) of this gene implicated in dehydrated hereditary stomatocytosis 1 and hereditary lymphedema. Orthologous to human PIEZO1 (piezo type mechanosensitive ion channel component 1 (Er blood group)). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Piezo-type mechanosensitive ion channel component 1 Protein FAM38A Piezo1 Fam38a |
| Molecular Weight(MW) | 292 kDa |
| Cellular Localization | Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Cell membrane, Cell projection, lamellipodium membrane. |
WB
Western blot analysis of FAM38A / PIEZO1 on different lysates with Rabbit anti-FAM38A / PIEZO1 antibody at 1/2,000 dilution. Lane 1: 293T transfected with mPiezo1 cell lysate, Lane 2: 293T transfected with mPiezo2 cell lysate (negative), Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of 293T cells labeling FAM38A / PIEZO1 with Rabbit anti-FAM38A / PIEZO1 antibody at 1/200 dilution. 293T cells, transfected with GFP-tagged mouse Piezo1 (top, positive) / tag-free mouse Piezo2 (bottom, negative) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FAM38A / PIEZO1 antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.| Application Notes | WB:1:2000 ICC/IF:1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
