WB
Human plasma was subjected to SDS PAGE followed by western blot with C3/C3b/C3c antibody at dilution of 1:60000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using C3/C3b/C3c antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using C3/C3b/C3c antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C3/C3b/C3c antibody at dilution of 1:200 and 488-Conjugated Goat Anti-Rabbit IgG(H+L).IF-P
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using C3/C3b/C3c antibody at dilution of 1:200 and 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).IP
IP result of anti-C3/C3b/C3c (IP:4ug; Detection: 1:1000) with human plasma lysate 4000 ug.| Product Name | C3/C3b/C3c Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human C3 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCIPWB |
| WB:1:20000-1:100000 IHC:1:1000-1:4000 ICC/IF:1:50-1:500 IF-P:1:50-1:500 IP: 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C3 |
|---|---|
| Gene Synonyms | ASP C3a C3b AHUS5 ARMD9 CPAMD1 HEL-S-62p |
| Gene Full Name | complement C3 |
| Gene Summary | Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients. [provided by RefSeq, Nov 2015] |
| Alternative Names | C3 C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 Complement C3 Complement component C3 CPAMD1 |
| Molecular Weight(MW) | 115 kDa, 70 kDa |
| Cellular Localization | Secreted |
WB
Human plasma was subjected to SDS PAGE followed by western blot with C3/C3b/C3c antibody at dilution of 1:60000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using C3/C3b/C3c antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using C3/C3b/C3c antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using C3/C3b/C3c antibody at dilution of 1:200 and 488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF-P
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using C3/C3b/C3c antibody at dilution of 1:200 and 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP
IP result of anti-C3/C3b/C3c (IP:4ug; Detection: 1:1000) with human plasma lysate 4000 ug.| Application Notes | WB:1:20000-1:100000 IHC:1:1000-1:4000 ICC/IF:1:50-1:500 IF-P:1:50-1:500 IP: 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
