WB
Western blot analysis of MAP4K1 / HPK1 on different lysates with Rabbit anti-MAP4K1 / HPK1 antibody at 1/2,000 dilution. Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-MAP4K1 / HPK1 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IP
MAP4K1 / HPK1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with MAP4K1 / HPK1 Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using MAP4K1 / HPK1 Recombinant Rabbit Monoclonal Antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP, VHH(HRP) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input). Lane 2: MAP4K1 / HPK1 Recombinant Rabbit Monoclonal Antibody IP in Jurkat cell lysate. Lane 3: Rabbit IgG instead of MAP4K1 / HPK1 Recombinant Rabbit Monoclonal Antibody in Jurkat cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 8 seconds.| Product Name | MAP4K1 / HPK1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human MAP4K1 aa 301-500. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:2000 IHC:1:2000 IP:1-2μg/sample |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAP4K1 |
|---|---|
| Gene Synonyms | HPK1 |
| Gene Full Name | mitogen-activated protein kinase kinase kinase kinase 1 |
| Gene Summary | Enables ATP binding activity and MAP kinase kinase kinase kinase activity. Involved in several processes, including JNK cascade; cellular response to phorbol 13-acetate 12-myristate; and protein phosphorylation. Located in membrane. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Hematopoietic progenitor kinase 1 antibody Hematopoietic progenitor kinase antibody HPK1 antibody Human Hematopoietic Progenitor Kinase antibody M4K1_HUMAN antibody Map4k1 antibody MAPK/ERK kinase kinase kinase 1 antibody MEK kinase kinase 1 antibody MEKKK 1 antibody Mitogen activated protein kinase kinase kinase kinase 1 antibody Mitogen-activated protein kinase kinase kinase kinase 1 antibody |
| Molecular Weight(MW) | 91 kDa(Observed band size:97 kDa) |
| Cellular Localization | Cytoplasm, membrane. |
WB
Western blot analysis of MAP4K1 / HPK1 on different lysates with Rabbit anti-MAP4K1 / HPK1 antibody at 1/2,000 dilution. Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-MAP4K1 / HPK1 antibody at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IP
MAP4K1 / HPK1 was immunoprecipitated from 0.2 mg Jurkat cell lysate with MAP4K1 / HPK1 Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using MAP4K1 / HPK1 Recombinant Rabbit Monoclonal Antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP, VHH(HRP) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: Jurkat cell lysate (input). Lane 2: MAP4K1 / HPK1 Recombinant Rabbit Monoclonal Antibody IP in Jurkat cell lysate. Lane 3: Rabbit IgG instead of MAP4K1 / HPK1 Recombinant Rabbit Monoclonal Antibody in Jurkat cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 8 seconds.| Application Notes | WB:1:2000 IHC:1:2000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
