WB
Western blot analysis of Beta III Tubulin on different lysates with Mouse anti-Beta III Tubulin antibody at 1/10,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 20 seconds; HeLa is a relative low expression control. and NIH/3T3 is a negative control. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/10,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperatureICC/IF
Application: Immunocytochemistry (IF-cell). Species: Human. Sample: Neuro-2a (Mouse brain neuroblastoma cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA601654, 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue).IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Cerebellum. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Beta III Tubulin Recombinant Antibody - Mouse IgG1 (Chimeric), 1/2,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Cerebellum. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Beta III Tubulin Recombinant Antibody - Mouse IgG1 (Chimeric), 1/2,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Cerebellum. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Beta III Tubulin Recombinant Antibody - Mouse IgG1 (Chimeric), 1/2,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IF-F
Application: Immunofluorescence (IHC-Fr). Species: Mouse. Tissue: Cerebral cortex. Sample: Frozen section. Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Beta III Tubulin Recombinant Antibody - Mouse IgG1 (Chimeric), 1/250, overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.IF-F
Application: Immunofluorescence (IHC-Fr). Species: Rat. Tissue: Cerebral cortex. Sample: Frozen section. Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Beta III Tubulin Recombinant Antibody - Mouse IgG1 (Chimeric), 1/250, overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.IF-P
Application: Immunofluorescence (IF-tissue). Species: Human. Tissue: Cerebellum. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Beta III Tubulin Recombinant Antibody - Mouse IgG1 (Chimeric), 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.IF-P
Application: Immunofluorescence (IF-tissue). Species: Mouse. Tissue: Cerebellum. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Beta III Tubulin Recombinant Antibody - Mouse IgG1 (Chimeric), 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.IF-P
Application: Immunofluorescence (IF-tissue). Species: Rat. Tissue: Cerebellum. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Beta III Tubulin Recombinant Antibody - Mouse IgG1 (Chimeric), 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.| Product Name | Beta III Tubulin Recombinant Antibody - Mouse IgG1 (Chimeric) |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within mouse Beta III Tubulin aa 401-450. |
| Isotype | IgG1 |
|---|---|
| Tested Applications | ICC/IFIF-FIF-PIHCWB |
| WB:1:10000 IHC:1:2000 ICC/IF:1:50 IF-F:1:200-1:500 IF-P:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TUBB3 |
|---|---|
| Gene Synonyms | CDCBM FEOM3 TUBB4 CDCBM1 CFEOM3 beta-4 CFEOM3A CFEOM3A1 |
| Gene Full Name | tubulin beta 3 class III |
| Gene Summary | This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010] |
| Alternative Names | beta 3 tubulin antibody beta 4 antibody beta-4 antibody CDCBM antibody CDCBM1 antibody CFEOM3 antibody CFEOM3A antibody FEOM3 antibody M(beta)3 antibody M(beta)6 antibody MC1R antibody Neuron specific beta III Tubulin antibody Neuron-specific class III beta-tubulin antibody QccE-11995 antibody QccE-15186 antibody TBB3_HUMAN antibody Tubb 3 antibody TUBB3 antibody TUBB4 antibody Tubulin beta 3 antibody Tubulin beta 3 chain antibody Tubulin beta 4 antibody Tubulin beta III antibody Tubulin beta-3 chain antibody Tubulin beta-4 chain antibody Tubulin beta-III antibody tuj 1 antibody tuj1 antibody |
| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Cytoplasm, cytoskeleton, growth cone, lamellipodium, filopodium. |
WB
Western blot analysis of Beta III Tubulin on different lysates with Mouse anti-Beta III Tubulin antibody at 1/10,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 20 seconds; HeLa is a relative low expression control. and NIH/3T3 is a negative control. Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/10,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Human. Sample: Neuro-2a (Mouse brain neuroblastoma cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: HA601654, 1/50, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/500, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Cerebellum. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Beta III Tubulin Recombinant Antibody - Mouse IgG1 (Chimeric), 1/2,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Cerebellum. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Beta III Tubulin Recombinant Antibody - Mouse IgG1 (Chimeric), 1/2,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Cerebellum. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: Beta III Tubulin Recombinant Antibody - Mouse IgG1 (Chimeric), 1/2,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IF-F
Application: Immunofluorescence (IHC-Fr). Species: Mouse. Tissue: Cerebral cortex. Sample: Frozen section. Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Beta III Tubulin Recombinant Antibody - Mouse IgG1 (Chimeric), 1/250, overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.
IF-F
Application: Immunofluorescence (IHC-Fr). Species: Rat. Tissue: Cerebral cortex. Sample: Frozen section. Antigen retrieval: Recommend. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Beta III Tubulin Recombinant Antibody - Mouse IgG1 (Chimeric), 1/250, overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.
IF-P
Application: Immunofluorescence (IF-tissue). Species: Human. Tissue: Cerebellum. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Beta III Tubulin Recombinant Antibody - Mouse IgG1 (Chimeric), 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.
IF-P
Application: Immunofluorescence (IF-tissue). Species: Mouse. Tissue: Cerebellum. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Beta III Tubulin Recombinant Antibody - Mouse IgG1 (Chimeric), 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.
IF-P
Application: Immunofluorescence (IF-tissue). Species: Rat. Tissue: Cerebellum. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: Beta III Tubulin Recombinant Antibody - Mouse IgG1 (Chimeric), 1/1,000, overnight at 4°C. Secondary antibody: Goat Anti-Mouse IgG (488), 1.5 hours at room temperature.| Application Notes | WB:1:10000 IHC:1:2000 ICC/IF:1:50 IF-F:1:200-1:500 IF-P:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
