WB
WB analysis using LDLR Polyclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with LDLR antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.IHC
IHC staining of human brain using LDLR Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human brain tissue slide using LDLR Antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).IP
IP experiment of HeLa using LDLR Polyclonal antibody. IP result of anti-LDLR (5ug; 1:500) with HeLa cells lysate 1200ug.ICC/IF
IF Staining of HeLa using LDLR Polyclonal antibody. Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LDLR antibody at dilution of 1:200 and 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Beta Actin antibody (red).| Product Name | LDLR Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human LDLR protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:2000-1:16000 IHC:1:200-1:800 ICC/IF:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LDLR |
|---|---|
| Gene Synonyms | LDLCQ2 |
| Gene Full Name | low density lipoprotein receptor |
| Gene Summary | The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. The encoded protein is normally bound at the cell membrane, where it binds low density lipoprotein/cholesterol and is taken into the cell. Lysosomes release the cholesterol, which is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2022] |
| Alternative Names | FH FHC LDL receptor |
| Molecular Weight(MW) | 95 kDa(Observed band size:100-160 kDa) |
| Cellular Localization | Cell membrane,Coated pit,Endosome,Golgi apparatus,LDL,Lysosome,Membrane. |
WB
WB analysis using LDLR Polyclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with LDLR antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC
IHC staining of human brain using LDLR Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human brain tissue slide using LDLR Antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).
IP
IP experiment of HeLa using LDLR Polyclonal antibody. IP result of anti-LDLR (5ug; 1:500) with HeLa cells lysate 1200ug.
ICC/IF
IF Staining of HeLa using LDLR Polyclonal antibody. Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LDLR antibody at dilution of 1:200 and 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Beta Actin antibody (red).| Application Notes | WB:1:2000-1:16000 IHC:1:200-1:800 ICC/IF:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20°C storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
