WB
Western blot analysis of lysates from wild type (WT) and LDL Receptor (LDLR) knockout (KO) HeLa cells using [KO Validated] LDL Receptor (LDLR) Rabbit pAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90 s.WB
Western blot analysis of various lysates using [KO Validated] LDL Receptor (LDLR) Rabbit pAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45 s.IHC
Immunohistochemistry analysis of paraffin embedded Mouse lung tissue using [KO Validated] LDL Receptor (LDLR) Rabbit pAb at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Rat brain tissue using [KO Validated] LDL Receptor (LDLR) Rabbit pAb at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.ICC/IF
Confocal imaging of Hep G2 cells using [KO Validated] LDL Receptor (LDLR) Rabbit pAb (dilution 1:300) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488 conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.| Product Name | LDL Receptor (LDLR) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:2000 - 1:5000 IHC:1:200 - 1:800 ICC/IF:1:100 - 1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LDLR |
|---|---|
| Gene Synonyms | LDLCQ2 |
| Gene Full Name | low density lipoprotein receptor |
| Gene Summary | The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. The encoded protein is normally bound at the cell membrane, where it binds low density lipoprotein/cholesterol and is taken into the cell. Lysosomes release the cholesterol, which is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2022] |
| Alternative Names | FH FHC FHCL1 LDLCQ2 LDL Receptor (LDLR) |
| Molecular Weight(MW) | 76-95 kDa(Observed band size:100-160 kDa) |
| Cellular Localization | Cell membrane, Cell surface, Early endosome, Endomembrane system, Golgi apparatus, Late endosome, Lysosome, Membrane, Single-pass type I membrane protein, clathrin-coated pit. |
WB
Western blot analysis of lysates from wild type (WT) and LDL Receptor (LDLR) knockout (KO) HeLa cells using [KO Validated] LDL Receptor (LDLR) Rabbit pAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90 s.
WB
Western blot analysis of various lysates using [KO Validated] LDL Receptor (LDLR) Rabbit pAb at 1:5000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45 s.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse lung tissue using [KO Validated] LDL Receptor (LDLR) Rabbit pAb at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Rat brain tissue using [KO Validated] LDL Receptor (LDLR) Rabbit pAb at a dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ICC/IF
Confocal imaging of Hep G2 cells using [KO Validated] LDL Receptor (LDLR) Rabbit pAb (dilution 1:300) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488 conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.| Application Notes | WB:1:2000 - 1:5000 IHC:1:200 - 1:800 ICC/IF:1:100 - 1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS, pH 7.3, containing 50% glycerol. Preserved with Proclin300 or sodium azide. May contain 0.05% BSA as specified on the Certificate of Analysis. |
