WB
Western blot analysis of anti-PRDX3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRDX3 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate .IHC
IHC analysis of PRDX3 using anti-PRDX3 antibody. PRDX3 was detected in a paraffin-embedded section of rat intestine tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of PRDX3 using anti-PRDX3 antibody. PRDX3 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of PRDX3 using anti-PRDX3 antibody. PRDX3 was detected in an immunocytochemical section of A549 cells. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of K562 cells using anti-PRDX3 antibody. Overlay histogram showing K562 cells stained with Rabbit polyclonal antibody to PRDX3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDX3 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to PRDX3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of mouse Peroxiredoxin 3, identical to the related rat sequence, and different from the related human sequence by three amino acids. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRDX3 |
|---|---|
| Gene Synonyms | AOP1 MER5 AOP-1 PPPCD SP-22 HBC189 SCAR32 PRO1748 prx-III |
| Gene Full Name | peroxiredoxin 3 |
| Gene Summary | This gene encodes a mitochondrial protein with antioxidant function. The protein is similar to the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase, and it can rescue bacterial resistance to alkylhydroperoxide in E. coli that lack the C22 subunit. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologs suggest that these genes consist of a family that is responsible for the regulation of cellular proliferation, differentiation and antioxidant functions. This family member can protect cells from oxidative stress, and it can promote cell survival in prostate cancer. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1, 3, 13 and 22. [provided by RefSeq, Oct 2014] |
| Alternative Names | Antioxidant protein 1 AOP 1 AOP1 HBC189 MER5 Peroxiredoxin 3 Peroxiredoxin III PRDX3 PRO1748 Protein MER5 homolog Prx III PRX3 SP 22 |
| Molecular Weight(MW) | 28 kDa(Observed band size:26 kDa ) |
| Cellular Localization | Cytoplasm,Endosome,Mitochondrion. |
WB
Western blot analysis of anti-PRDX3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PRDX3 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate .
IHC
IHC analysis of PRDX3 using anti-PRDX3 antibody. PRDX3 was detected in a paraffin-embedded section of rat intestine tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of PRDX3 using anti-PRDX3 antibody. PRDX3 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of PRDX3 using anti-PRDX3 antibody. PRDX3 was detected in an immunocytochemical section of A549 cells. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of K562 cells using anti-PRDX3 antibody. Overlay histogram showing K562 cells stained with Rabbit polyclonal antibody to PRDX3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRDX3 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
