WB
Western blot analysis of Leukotriene B4 Receptor on different lysates with Rabbit anti-Leukotriene B4 Receptor antibody at 1/1,000 dilution. Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Leukotriene B4 Receptor antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Product Name | Leukotriene B4 Receptor Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human Leukotriene B4 Receptor aa 303-352 / 352. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LTB4R |
|---|---|
| Gene Synonyms | BLT1 BLTR P2Y7 GPR16 LTBR1 P2RY7 CMKRL1 LTB4R1 |
| Gene Full Name | leukotriene B4 receptor |
| Gene Summary | Predicted to enable G protein-coupled peptide receptor activity and leukotriene B4 receptor activity. Predicted to be involved in neuropeptide signaling pathway. Predicted to act upstream of or within signal transduction. Predicted to be located in membrane. Predicted to be active in plasma membrane. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | BLT antibody BLT1 antibody BLTR antibody Chemoattractant receptor-like 1 antibody Chemokine receptor like 1 antibody CMKRL1 antibody G protein coupled receptor 16 antibody G-protein coupled receptor 16 antibody GPR16 antibody Leukotriene B4 G Protein coupled receptor antibody Leukotriene B4 receptor 1 antibody Leukotriene B4 receptor antibody LT4R1_HUMAN antibody LTB4 R 1 antibody LTB4-R 1 antibody LTB4-R1 antibody LTB4R 1 antibody Ltb4r antibody LTB4R1 antibody LTBR1 antibody P2RY7 antibody P2Y purinoceptor 7 antibody P2Y7 antibody Purinergic receptor P2Y G protein coupled 7 antibody |
| Molecular Weight(MW) | 38 kDa |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of Leukotriene B4 Receptor on different lysates with Rabbit anti-Leukotriene B4 Receptor antibody at 1/1,000 dilution. Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Leukotriene B4 Receptor antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
