WB
Western blot analysis of lysates from various samples, using PTPN12 Ab. Lane 1: HepG2 cells(heat-shock treatment), blocked with antigen-specific peptides. Lane 2: HepG2 cells(heat-shock treatment). Lane 3: Hela cells(heat-shock treatment).IHC
Rabbit polyclonal antibody to PTPN12 at 1/100 staining rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-rabbit Ab was used as the secondary Ab.ICC/IF
Rabbit polyclonal antibody to PTPN12 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).| Product Name | Rabbit polyclonal antibody to PTPN12 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human PTPN12. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTPN12 |
|---|---|
| Gene Synonyms | PTPG1 PTP-PEST |
| Gene Full Name | protein tyrosine phosphatase non-receptor type 12 |
| Gene Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may regulate protein intracellular half-life. This PTP was found to bind and dephosphorylate the product of the oncogene c-ABL and thus may play a role in oncogenesis. This PTP was also shown to interact with, and dephosphorylate, various products related to cytoskeletal structure and cell adhesion, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin. This suggests it has a regulatory role in controlling cell shape and mobility. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| Alternative Names | Protein tyrosine phosphatase G1 Protein tyrosine phosphatase non receptor type 12 Protein-tyrosine phosphatase G1 PTN12_HUMAN PTP PEST PTP-PEST PTPG1 Ptpn12 Tyrosine protein phosphatase non receptor type 12 Tyrosine-protein phosphatase non-receptor type 12 |
| Molecular Weight(MW) | 88 kDa |
| Cellular Localization | Cytoplasm. Cell junction>Focal adhesion. Cell projection>Podosome. |
WB
Western blot analysis of lysates from various samples, using PTPN12 Ab. Lane 1: HepG2 cells(heat-shock treatment), blocked with antigen-specific peptides. Lane 2: HepG2 cells(heat-shock treatment). Lane 3: Hela cells(heat-shock treatment).
IHC
Rabbit polyclonal antibody to PTPN12 at 1/100 staining rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-rabbit Ab was used as the secondary Ab.
ICC/IF
Rabbit polyclonal antibody to PTPN12 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
