WB
Western blot analysis of PUF60 using anti-PUF60 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PUF60 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of PUF60 using anti-PUF60 antibody. PUF60 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-PUF60 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IP
IP analysis of PUF60 using anti-PUF60 antibody in MCF-7 whole cell lysate. Western blot analysis of PUF60 using anti- PUF60 antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- PUF60 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.FC
Flow Cytometry analysis of Caco-2 cells using anti-PUF60 antibody. Overlay histogram showing Caco-2 cells stained with anti-PUF60 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PUF60 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to PUF60 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human PUF60 recombinant protein (Position: Q48-A559). Human PUF60 shares 98.8% amino acid (aa) sequence identity with both mouse and rat PUF60. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCIPWB |
| WB:1:1000-1:5000 IHC:1:50-1:400 FC:1:50-1:200 IP:1:250-1:300 |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PUF60 |
|---|---|
| Gene Synonyms | FIR VRJS RoBPI SIAHBP1 |
| Gene Full Name | poly(U) binding splicing factor 60 |
| Gene Summary | This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012] |
| Alternative Names | FBP interacting repressor FIR PUF60 Ro binding protein 1 RoBP1 RoBPI Siah binding protein 1 Siah BP1 SIAHBP1 |
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of PUF60 using anti-PUF60 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PUF60 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of PUF60 using anti-PUF60 antibody. PUF60 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-PUF60 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IP
IP analysis of PUF60 using anti-PUF60 antibody in MCF-7 whole cell lysate. Western blot analysis of PUF60 using anti- PUF60 antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- PUF60 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
FC
Flow Cytometry analysis of Caco-2 cells using anti-PUF60 antibody. Overlay histogram showing Caco-2 cells stained with anti-PUF60 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PUF60 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:400 FC:1:50-1:200 IP:1:250-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
