WB
Western blot analysis of p63/TP63 using anti-p63/TP63 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-p63/TP63 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of p63/TP63 using anti-p63/TP63 antibody. p63/TP63 was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-p63/TP63 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of p63/TP63 using anti-p63/TP63 antibody. p63/TP63 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-p63/TP63 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of A431 cells using anti-p63/TP63 antibody. Overlay histogram showing A431 cells stained with Rabbit polyclonal antibody to p63/TP63 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p63/TP63 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.IF-P
IF analysis of p63/TP63 using anti-p63/TP63 antibody. p63/TP63 was detected in a paraffin-embedded section of human prostate hyperplasia tissue. The tissue section was incubated with rabbit anti-p63/TP63 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody to p63/TP63 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human p63 recombinant protein (Position: S311-E680). Human p63 shares 98% amino acid (aa) sequence identity with both mouse and rat p63. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TP63 |
|---|---|
| Gene Synonyms | AIS KET LMS NBP RHS p40 p51 p63 EEC3 OFC8 p73H p73L SHFM4 TP53L TP73L p53CP TP53CP B(p51A) B(p51B) |
| Gene Full Name | tumor protein p63 |
| Gene Summary | This gene encodes a member of the p53 family of transcription factors. The functional domains of p53 family proteins include an N-terminal transactivation domain, a central DNA-binding domain and an oligomerization domain. Alternative splicing of this gene and the use of alternative promoters results in multiple transcript variants encoding different isoforms that vary in their functional properties. These isoforms function during skin development and maintenance, adult stem/progenitor cell regulation, heart development and premature aging. Some isoforms have been found to protect the germline by eliminating oocytes or testicular germ cells that have suffered DNA damage. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. [provided by RefSeq, Aug 2016] |
| Alternative Names | AIS B(p51A) B(p51B) CUSP EEC3 KET LMS NBP OFC8 p40 p51 p53CP p63 p73H p73L RHS SHFM4 TP53CP TP53L TP63 TP73L Tumor protein 63 tumor protein p63 Tumor protein p73 like |
| Molecular Weight(MW) | 77 kDa(Observed band size:75 kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of p63/TP63 using anti-p63/TP63 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-p63/TP63 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of p63/TP63 using anti-p63/TP63 antibody. p63/TP63 was detected in a paraffin-embedded section of human oesophagus squama cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-p63/TP63 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of p63/TP63 using anti-p63/TP63 antibody. p63/TP63 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-p63/TP63 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of A431 cells using anti-p63/TP63 antibody. Overlay histogram showing A431 cells stained with Rabbit polyclonal antibody to p63/TP63 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p63/TP63 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF-P
IF analysis of p63/TP63 using anti-p63/TP63 antibody. p63/TP63 was detected in a paraffin-embedded section of human prostate hyperplasia tissue. The tissue section was incubated with rabbit anti-p63/TP63 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
