WB
Western blot analysis of RBPMS using anti-RBPMS antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were traRBPMSerred to a membrane. Then the membrane was incubated with rabbit anti-RBPMS antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The siRBPMS is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of RBPMS using anti-RBPMS antibody. RBPMS was detected in a paraffin-embedded section of human cardiac muscle tissue. The tissue section was incubated with rabbit anti-RBPMS Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of RBPMS using anti-RBPMS antibody. RBPMS was detected in a paraffin-embedded section of mouse heart tissue. The tissue section was incubated with rabbit anti-RBPMS Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of RBPMS using anti-RBPMS antibody. RBPMS was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-RBPMS Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.FC
Flow Cytometry analysis of Caco-2 cells using anti-RBPMS antibody. Overlay histogram showing Caco-2 cells stained with Rabbit polyclonal antibody to RBPMS (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBPMS Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to RBPMS |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human RBPMS recombinant protein (Position: K6-C196). Human RBPMS shares 99.4% amino acid (aa) sequence identity with both mouse and rat RBPMS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:1000-1:5000 IHC:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RBPMS |
|---|---|
| Gene Synonyms | HERMES |
| Gene Full Name | RNA binding protein, mRNA processing factor |
| Gene Summary | This gene encodes a member of the RNA recognition motif family of RNA-binding proteins. The RNA recognition motif is between 80-100 amino acids in length and family members contain one to four copies of the motif. The RNA recognition motif consists of two short stretches of conserved sequence, as well as a few highly conserved hydrophobic residues. The encoded protein has a single, putative RNA recognition motif in its N-terminus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013] |
| Alternative Names | HERMES RBP MS RBPMS |
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Western blot analysis of RBPMS using anti-RBPMS antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were traRBPMSerred to a membrane. Then the membrane was incubated with rabbit anti-RBPMS antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The siRBPMS is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of RBPMS using anti-RBPMS antibody. RBPMS was detected in a paraffin-embedded section of human cardiac muscle tissue. The tissue section was incubated with rabbit anti-RBPMS Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of RBPMS using anti-RBPMS antibody. RBPMS was detected in a paraffin-embedded section of mouse heart tissue. The tissue section was incubated with rabbit anti-RBPMS Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of RBPMS using anti-RBPMS antibody. RBPMS was detected in a paraffin-embedded section of rat heart tissue. The tissue section was incubated with rabbit anti-RBPMS Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
FC
Flow Cytometry analysis of Caco-2 cells using anti-RBPMS antibody. Overlay histogram showing Caco-2 cells stained with Rabbit polyclonal antibody to RBPMS (Blue line). To facilitate intrMyelin basic protein/MBPllular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBPMS Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
