ICC/IF
HepG2-NTCP HBV infected; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (HBcAg) Polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (1:200) at 37°C for 90 minutes, DAPI (blue)was used to stain the cell nuclei. Maria Francesca Cortese,PhD researcher in Vall d'Hebron Research Institute (VHIR) of Barcelona (Spain).| Product Name | HBV | HBcAg Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from Hepatitis B Virus Core Antigen : 1-100/185. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| ICC/IF:1:50-1:200 |
|
| Species Reactivity | Virus |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | S |
|---|---|
| Alternative Names | HBSAG_HBVE1 S L glycoprotein L-HBsAg (LHB) Large S protein Large surface protein Major surface antigen |
| Molecular Weight(MW) | 21 kDa |
| Function | The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assumes fusion between virion membrane and endosomal membrane (Probable). In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein. The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid. |
| Cellular Localization | Membrane,Viral envelope protein,Virion. |
ICC/IF
HepG2-NTCP HBV infected; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (HBcAg) Polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (1:200) at 37°C for 90 minutes, DAPI (blue)was used to stain the cell nuclei. Maria Francesca Cortese,PhD researcher in Vall d'Hebron Research Institute (VHIR) of Barcelona (Spain).| Application Notes | ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
