WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cleaved Caspase-3 (Asp175) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human colon carcinoma was stained with anti-Cleaved Caspase-3 (Asp175) Rabbit antibody.ICC/IF
Immunofluorescence analysis of HEK293. Picture A: Cleaved Caspase-3 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | Cleaved Caspase-3(Asp175) Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CASP3 |
|---|---|
| Gene Synonyms | CPP32 SCA-1 CPP32B |
| Gene Full Name | caspase 3 |
| Gene Summary | The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. [provided by RefSeq, Aug 2017] |
| Alternative Names | CASP3 CPP32 Caspase-3 CASP-3 Apopain Cysteine protease CPP32 CPP-32 Protein Yama SREBP cleavage activity 1 SCA-1 |
| Molecular Weight(MW) | 17 kDa,19 kDa(Observed band size:35 kDa(Full Length),19 kDa(p19),17 kDa(p17)) |
| Cellular Localization | Cytoplasm. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-Cleaved Caspase-3 (Asp175) antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human colon carcinoma was stained with anti-Cleaved Caspase-3 (Asp175) Rabbit antibody.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: Cleaved Caspase-3 antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
