WB
Western blot analysis of various lysates using CX3CR1 Rabbit pAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.IHC
Immunohistochemistry analysis of paraffin embedded Human colon carcinoma using CX3CR1 Rabbit pAb at dilution of 1:20 (40x lens). Microwave antigen retrieval performed with 0.05M Tris/EDTA Buffer (pH 8.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin embedded Rat spleen using CX3CR1 Rabbit pAb at dilution of 1:20 (40x lens). Microwave antigen retrieval performed with 0.05M Tris/EDTA Buffer (pH 8.0) prior to IHC staining.| Product Name | CX3CR1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500 - 1:1000 IHC:1:50 - 1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CX3CR1 |
|---|---|
| Gene Synonyms | V28 CCRL1 GPR13 CMKDR1 GPRV28 CMKBRL1 |
| Gene Full Name | C-X3-C motif chemokine receptor 1 |
| Gene Summary | Fractalkine is a transmembrane protein and chemokine involved in the adhesion and migration of leukocytes. The protein encoded by this gene is a receptor for fractalkine. The encoded protein also is a coreceptor for HIV-1, and some variations in this gene lead to increased susceptibility to HIV-1 infection and rapid progression to AIDS. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2010] |
| Alternative Names | V28 CCRL1 GPR13 CMKDR1 GPRV28 CMKBRL1 CX3CR1 |
| Molecular Weight(MW) | 40 kDa(Observed band size: 52kDa) |
| Cellular Localization | Cell membrane, Multi-pass membrane protein. |
WB
Western blot analysis of various lysates using CX3CR1 Rabbit pAb at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
IHC
Immunohistochemistry analysis of paraffin embedded Human colon carcinoma using CX3CR1 Rabbit pAb at dilution of 1:20 (40x lens). Microwave antigen retrieval performed with 0.05M Tris/EDTA Buffer (pH 8.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin embedded Rat spleen using CX3CR1 Rabbit pAb at dilution of 1:20 (40x lens). Microwave antigen retrieval performed with 0.05M Tris/EDTA Buffer (pH 8.0) prior to IHC staining.| Application Notes | WB:1:500 - 1:1000 IHC:1:50 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
