WB
Western blot analysis of PTPLA expression in MDAMB231 (A) whole cell lysates.IHC
Immunohistochemical analysis of PTPLA staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | Anti-PTPLA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human PTPLA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HACD1 |
|---|---|
| Gene Synonyms | CAP MYONP PTPLA CMYO11 CMYP11 |
| Gene Full Name | 3-hydroxyacyl-CoA dehydratase 1 |
| Gene Summary | The protein encoded by this gene contains a characteristic catalytic motif of the protein tyrosine phosphatases (PTPs) family. The PTP motif of this protein has the highly conserved arginine residue replaced by a proline residue; thus it may represent a distinct class of PTPs. Members of the PTP family are known to be signaling molecules that regulate a variety of cellular processes. This gene was preferentially expressed in both adult and fetal heart. A much lower expression level was detected in skeletal and smooth muscle tissues, and no expression was observed in other tissues. The tissue specific expression in the developing and adult heart suggests a role in regulating cardiac development and differentiation. [provided by RefSeq, Jul 2008] |
| Alternative Names | HACD1 Very-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase 1 3-hydroxyacyl-CoA dehydratase 1 HACD1 Cementum attachment protein Protein-tyrosine phosphatase-like member A |
| Molecular Weight(MW) | 32 kDa(Observed band size: 38 kDa) |
| Cellular Localization | Endoplasmic reticulum,Membrane. |
WB
Western blot analysis of PTPLA expression in MDAMB231 (A) whole cell lysates.
IHC
Immunohistochemical analysis of PTPLA staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
