WB
Western blot analysis of anti- SNX1 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Use rabbit anti- SNX1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.IHC
IHC analysis of SNX1 using anti-SNX1 antibody. SNX1 was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of SNX1 using anti-SNX1 antibody. SNX1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-SNX1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of U2OS cells using anti-SNX1 antibody. Overlay histogram showing U2OS cells stained with Anti-SNX1 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNX1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to SNX1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human SNX1 recombinant protein (Position: Q83-Q505). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNX1 |
|---|---|
| Gene Synonyms | VPS5 HsT17379 |
| Gene Full Name | sorting nexin 1 |
| Gene Summary | This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. This protein also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members. This gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
| Alternative Names | HsT17379 SNX1 SNX1A sorting nexin 1 Vps5 |
| Molecular Weight(MW) | 59 kDa(Observed band size:70-75 kDa) |
| Cellular Localization | Cell projection,Endosome,Golgi apparatus,Membrane. |
WB
Western blot analysis of anti- SNX1 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Use rabbit anti- SNX1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit.
IHC
IHC analysis of SNX1 using anti-SNX1 antibody. SNX1 was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of SNX1 using anti-SNX1 antibody. SNX1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-SNX1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of U2OS cells using anti-SNX1 antibody. Overlay histogram showing U2OS cells stained with Anti-SNX1 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SNX1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
