WB
Western blot analysis of CLIC4 on different lysates with Rabbit anti-CLIC4 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane), Lane 2: JAR cell lysate (20 µg/Lane), Lane 3: HepG2 cell lysate (20 µg/Lane), Lane 4: RAW264.7 cell lysate (20 µg/Lane), Lane 5: Neuro-2a cell lysate (20 µg/Lane), Lane 6: C6 cell lysate (20 µg/Lane), Lane 7: Human kidney tissue lysate (40 µg/Lane), Lane 8: Rat kidney tissue lysate (40 µg/Lane), Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of HeLa cells labeling CLIC4 with Rabbit anti-CLIC4 antibody at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CLIC4 antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L(488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.FC
Flow cytometric analysis of HeLa cells labeling CLIC4. Cells were fixed and permeabilized. Then stained with the primary antibody (1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | CLIC4 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human CLIC4 aa 1-50 / 253. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:1000 ICC/IF:1:200 FC:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CLIC4 |
|---|---|
| Gene Synonyms | H1 huH1 p64H1 CLIC4L MTCLIC |
| Gene Full Name | CLIC family member 4 |
| Gene Summary | Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. Chloride intracellular channel 4 (CLIC4) protein, encoded by the CLIC4 gene, is a member of the p64 family; the gene is expressed in many tissues and exhibits a intracellular vesicular pattern in Panc-1 cells (pancreatic cancer cells). [provided by RefSeq, Jul 2008] |
| Alternative Names | Chloride intracellular channel 4 antibody Chloride intracellular channel 4 (mitochondrial) antibody Chloride intracellular channel 4 like antibody Chloride intracellular channel protein 4 antibody Clic4 antibody CLIC4_HUMAN antibody CLIC4L antibody DKFZP566G223 antibody FLJ38640 antibody H1 antibody HUH1 antibody Intracellular chloride ion channel protein p64H1 antibody MC3S5 antibody mtCLIC antibody p64H1 antibody |
| Molecular Weight(MW) | 29 kDa |
| Cellular Localization | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasmic vesicle membrane, Nucleus, Cell membrane, Mitochondrion, Cell junction. |
WB
Western blot analysis of CLIC4 on different lysates with Rabbit anti-CLIC4 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane), Lane 2: JAR cell lysate (20 µg/Lane), Lane 3: HepG2 cell lysate (20 µg/Lane), Lane 4: RAW264.7 cell lysate (20 µg/Lane), Lane 5: Neuro-2a cell lysate (20 µg/Lane), Lane 6: C6 cell lysate (20 µg/Lane), Lane 7: Human kidney tissue lysate (40 µg/Lane), Lane 8: Rat kidney tissue lysate (40 µg/Lane), Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of HeLa cells labeling CLIC4 with Rabbit anti-CLIC4 antibody at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CLIC4 antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L(488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
FC
Flow cytometric analysis of HeLa cells labeling CLIC4. Cells were fixed and permeabilized. Then stained with the primary antibody (1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 ICC/IF:1:200 FC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
