WB
Western blot analysis of extracts of various cell lines, using ARID1B antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.IHC
Immunohistochemistry of paraffin-embedded Mouse brain using ARID1B Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.IHC
Immunohistochemistry of paraffin-embedded Rat heart using ARID1B Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.IHC
Immunohistochemistry of paraffin-embedded Human breast using ARID1B Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.ICC/IF
| Product Name | ARID1B polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein of human ARID1B(NP_059989.2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:200 - 1:2000 IHC:1:50 - 1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARID1B |
|---|---|
| Gene Synonyms | CSS1 OSA2 6A3-5 DAN15 MRD12 P250R BRIGHT BAF250B SMARCF2 ELD/OSA1 |
| Gene Full Name | AT-rich interaction domain 1B |
| Gene Summary | This locus encodes an AT-rich DNA interacting domain-containing protein. The encoded protein is a component of the SWI/SNF chromatin remodeling complex and may play a role in cell-cycle activation. The protein encoded by this locus is similar to AT-rich interactive domain-containing protein 1A. These two proteins function as alternative, mutually exclusive ARID-subunits of the SWI/SNF complex. The associated complexes play opposing roles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
| Alternative Names | ARID1B 6A3-5 BAF250B BRIGHT CSS1 DAN15 ELD/OSA1 MRD12 OSA2 P250R |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of extracts of various cell lines, using ARID1B antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.
IHC
Immunohistochemistry of paraffin-embedded Mouse brain using ARID1B Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
IHC
Immunohistochemistry of paraffin-embedded Rat heart using ARID1B Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
IHC
Immunohistochemistry of paraffin-embedded Human breast using ARID1B Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ICC/IF
| Application Notes | WB:1:200 - 1:2000 IHC:1:50 - 1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. |
