WB
Western blot analysis of IGF1R on different lysates with Rabbit anti-IGF1R antibody at 1/20,000 dilution. The expression profile observed is consistent with what has been described in the literature. Lysates/proteins at 15 µg/Lane. Exposure time: 25 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: IGF1R Recombinant Rabbit Monoclonal Antibody, 1/20,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Bronchus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: IGF1R Recombinant Rabbit Monoclonal Antibody, 1/2,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Kidney. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: IGF1R Recombinant Rabbit Monoclonal Antibody, 1/2,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Kidney. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: IGF1R Recombinant Rabbit Monoclonal Antibody, 1/2,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Human. Sample: MCF7 (Human breast cancer cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: IGF1R Recombinant Rabbit Monoclonal Antibody, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).FC
Application: Flow Cytometry. Species: Human. Sample: MCF7 (Human breast cancer cells). Blocking: 1% BSA + 10% normal goat serum + human FcR (Miltenyi Biotec), 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: IGF1R Recombinant Rabbit Monoclonal Antibody (1/1,000, Red) compared with Rabbit IgG Isotype Control (Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (488), 15 minutes at room temperature.| Product Name | IGF1R Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human IGF1R aa 550-935. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:5000-1:20000 IHC:1:2000 ICC/IF:1:200 FC:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IGF1R |
|---|---|
| Gene Synonyms | IGFR CD221 IGFIR JTK13 |
| Gene Full Name | insulin like growth factor 1 receptor |
| Gene Summary | This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014] |
| Alternative Names | Insulin-like growth factor 1 receptor beta chain antibody CD221 antibody CD221 antigen antibody IGF 1 receptor antibody IGF 1R antibody IGF I receptor antibody IGF-I receptor antibody Igf1r antibody IGF1R_HUMAN antibody IGFIR antibody IGFIRC antibody IGFR antibody Insulin like growth factor 1 receptor antibody Insulin like growth factor 1 receptor precursor antibody Insulin-like growth factor 1 receptor beta chain antibody Insulin-like growth factor I receptor antibody JTK13 antibody MGC142170 antibody MGC142172 antibody MGC18216 antibody Soluble IGF1R variant 1 antibody Soluble IGF1R variant 2 antibody |
| Molecular Weight(MW) | 155 kDa(Observed band size: 100/200 kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of IGF1R on different lysates with Rabbit anti-IGF1R antibody at 1/20,000 dilution. The expression profile observed is consistent with what has been described in the literature. Lysates/proteins at 15 µg/Lane. Exposure time: 25 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: IGF1R Recombinant Rabbit Monoclonal Antibody, 1/20,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Bronchus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: IGF1R Recombinant Rabbit Monoclonal Antibody, 1/2,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Kidney. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: IGF1R Recombinant Rabbit Monoclonal Antibody, 1/2,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Kidney. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: IGF1R Recombinant Rabbit Monoclonal Antibody, 1/2,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Human. Sample: MCF7 (Human breast cancer cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: IGF1R Recombinant Rabbit Monoclonal Antibody, 1/200, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
FC
Application: Flow Cytometry. Species: Human. Sample: MCF7 (Human breast cancer cells). Blocking: 1% BSA + 10% normal goat serum + human FcR (Miltenyi Biotec), 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: IGF1R Recombinant Rabbit Monoclonal Antibody (1/1,000, Red) compared with Rabbit IgG Isotype Control (Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (488), 15 minutes at room temperature.| Application Notes | WB:1:5000-1:20000 IHC:1:2000 ICC/IF:1:200 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
