WB
25 ug total protein per lane of various lysates (see on figure) probed with GPR30 polyclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.IHC
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GPR30 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by incubation with HRP-conjugated secondary antibody and DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GPR30 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by incubation with HRP-conjugated secondary antibody and DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GPR30 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by incubation with HRP-conjugated secondary antibody and DAB.IF-P
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GPR30 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.IF-P
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GPR30 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.IF-P
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GPR30 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.| Product Name | GPER1 | GPR30 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human GPR30 : 251-375/375 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 IF-P:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GPER1 |
|---|---|
| Gene Synonyms | mER CEPR GPER DRY12 FEG-1 GPR30 LERGU LyGPR CMKRL2 LERGU2 GPCR-Br |
| Gene Full Name | G protein-coupled estrogen receptor 1 |
| Gene Summary | This gene encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum and a member of the G-protein coupled receptor 1 family. This receptor binds estrogen and activates multiple downstream signaling pathways, leading to stimulation of adenylate cyclase and an increase in cyclic AMP levels, while also promoting intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. This receptor has been shown to play a role in diverse biological processes, including bone and nervous system development, metabolism, cognition, male fertility and uterine function. [provided by RefSeq, Aug 2017] |
| Alternative Names | CEPR CMKRL2 DRY12 FEG-1 GPCR-Br GPER GPR30 LERGU LERGU2 LyGPR mER 6330420K13Rik Ceprl GPR41 GPER1_HUMAN GPER1 Chemoattractant receptor-like 2 Flow-induced endothelial G-protein coupled receptor 1 (FEG-1) G protein-coupled estrogen receptor 1 G-protein coupled receptor 30 IL8-related receptor DRY12 Lymphocyte-derived G-protein coupled receptor (LYGPR) Membrane estrogen receptor (mER) GPER1_MOUSE GPER1_RAT G-protein coupled receptor 41 G protein-coupled receptor 30 |
| Molecular Weight(MW) | 42 kDa(Observed band size: 58 kDa) |
| Cellular Localization | Cell membrane,Cell projection,Cytoplasm,Cytoplasmic vesicle,Cytoskeleton,Endoplasmic reticulum,Endosome ,Golgi apparatus,Membrane,Mitochondrion,Nucleus,Postsynaptic cell membrane,Synapse. |
WB
25 ug total protein per lane of various lysates (see on figure) probed with GPR30 polyclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
IHC
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GPR30 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by incubation with HRP-conjugated secondary antibody and DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GPR30 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by incubation with HRP-conjugated secondary antibody and DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GPR30 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by incubation with HRP-conjugated secondary antibody and DAB.
IF-P
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GPR30 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.
IF-P
Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GPR30 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.
IF-P
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with GPR30 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated Goat Anti-Rabbit IgG antibody (Red), DAPI (blue) was used to stain the cell nuclei.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 IF-P:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
