WB
Western blot analysis of NID1 using anti-NID1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NID1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of NID1 using anti-NID1 antibody. NID1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-NID1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis of NID1 using anti-NID1 antibody. NID1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-NID1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of HepG2 cells using anti-NID1 antibody. Overlay histogram showing HepG2 cells stained with Anti-Entactin/NID1 Antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NID1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to Entactin/NID1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human Entactin/NID1 recombinant protein (Position: R31-H1209). Human Entactin/NID1 shares 85.6% amino acid (aa) sequence identity with mouse Entactin/NID1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NID1 |
|---|---|
| Gene Synonyms | NID |
| Gene Full Name | nidogen 1 |
| Gene Summary | This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq, Jul 2008] |
| Alternative Names | NID1 NID NID 1 NID-1 nidogen 1 |
| Molecular Weight(MW) | 136 kDa(Observed band size: 150 kDa) |
| Cellular Localization | Basement membrane,Extracellular matrix,Secreted. |
WB
Western blot analysis of NID1 using anti-NID1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NID1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of NID1 using anti-NID1 antibody. NID1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-NID1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis of NID1 using anti-NID1 antibody. NID1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-NID1 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of HepG2 cells using anti-NID1 antibody. Overlay histogram showing HepG2 cells stained with Anti-Entactin/NID1 Antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-NID1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
