WB
Western blot analysis of lysates from HT-29, LOVO, and A549 cells, using S1P Receptor EDG1 Antibody. The lane on the right is blocked with the synthesized peptide.ICC/IF
Immunofluorescence analysis of A549 cells, using S1P Receptor EDG1 Antibody. The picture on the right is blocked with the synthesized peptide.| Product Name | EDG-1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human S1P Receptor EDG1. AA range:206-255. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | S1PR1 |
|---|---|
| Gene Synonyms | EDG1 S1P1 CD363 ECGF1 EDG-1 CHEDG1 D1S3362 |
| Gene Full Name | sphingosine-1-phosphate receptor 1 |
| Gene Summary | The protein encoded by this gene is structurally similar to G protein-coupled receptors and is highly expressed in endothelial cells. It binds the ligand sphingosine-1-phosphate with high affinity and high specificity, and suggested to be involved in the processes that regulate the differentiation of endothelial cells. Activation of this receptor induces cell-cell adhesion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
| Alternative Names |
S1PR1 CHEDG1 EDG1 Sphingosine 1-phosphate receptor 1 S1P receptor 1 S1P1 Endothelial differentiation G-protein coupled receptor 1 Sphingosine 1-phosphate receptor Edg-1 S1P receptor Edg-1 CD antigen CD363 |
| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Cell membrane ; Multi-pass membrane protein. Endosome. Membrane raft. Recruited to caveolin-enriched plasma membrane microdomains in response to oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine. Ligand binding leads to receptor internalization. |
WB
Western blot analysis of lysates from HT-29, LOVO, and A549 cells, using S1P Receptor EDG1 Antibody. The lane on the right is blocked with the synthesized peptide.
ICC/IF
Immunofluorescence analysis of A549 cells, using S1P Receptor EDG1 Antibody. The picture on the right is blocked with the synthesized peptide.| Application Notes | WB:1:500-1:2000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
