WB
Sample: U937(Human) Cell Lysate at 30 ug. Primary: Anti-CD80 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.IHC
Paraformaldehyde-fixed, paraffin embedded (Human melanoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD80) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.| Product Name | CD80 | B7-1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human CD80 : 201-300/288 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CD80 |
|---|---|
| Gene Synonyms | B7 BB1 B7-1 B7.1 LAB7 CD28LG CD28LG1 |
| Gene Full Name | CD80 molecule |
| Gene Summary | The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011] |
| Alternative Names | B7 B7-1 B7.1 BB1 CD28LG CD28LG1 LAB7 B71 Cd28l Ly-53 Ly53 MIC17 TSA1 CD80_HUMAN CD80 Activation B7-1 antigen CTLA-4 counter-receptor B7.1 (B7) CD80_MOUSE Activation B7-1 antigen (B7) CD80 molecule CD80 antigen (CD28 antigen ligand 1 B7-1 antigen) B-lymphocyte activation antigen B7 |
| Molecular Weight(MW) | 32 kDa |
| Cellular Localization | Cell membrane,Membrane. |
WB
Sample: U937(Human) Cell Lysate at 30 ug. Primary: Anti-CD80 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.
IHC
Paraformaldehyde-fixed, paraffin embedded (Human melanoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD80) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
