WB
Western blot analysis of anti-CSNK2B antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CSNK2B antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of CSNK2B using anti-CSNK2B antibody. CSNK2B was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-CSNK2B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of CSNK2B using anti-CSNK2B antibody. CSNK2B was detected in a paraffin-embedded section of rat testis tissue. The tissue section was incubated with rabbit anti-CSNK2B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Rabbit monoclonal antibody to CSNK2B |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human Casein Kinase 2 beta Participates in Wnt signaling (By similarity) . Plays a complex role in regulating the basal catalytic activity of the alpha subunit. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CSNK2B |
|---|---|
| Gene Synonyms | G5A CK2B CK2N Ckb1 Ckb2 CSK2B POBINDS CK II beta |
| Gene Full Name | casein kinase 2 beta |
| Gene Summary | This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013] |
| Alternative Names | Casein kinase II subunit beta CK II beta CK2B CK2N CSK2B CSNK2B CSNK2B-specific G5A Phosvitin Protein G5a |
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of anti-CSNK2B antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CSNK2B antigen affinity purified monoclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of CSNK2B using anti-CSNK2B antibody. CSNK2B was detected in a paraffin-embedded section of human colon tissue. The tissue section was incubated with rabbit anti-CSNK2B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of CSNK2B using anti-CSNK2B antibody. CSNK2B was detected in a paraffin-embedded section of rat testis tissue. The tissue section was incubated with rabbit anti-CSNK2B Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml; Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide, 0.4-0.5 mg/ml BSA and 50% glycerol. |
