WB
Western blot analysis of ATP5F1A using anti-ATP5F1A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATP5F1A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ATP5F1A using anti-ATP5F1A antibody. ATP5F1A was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATP5F1A Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of ATP5F1A using anti-ATP5F1A antibody. ATP5F1A was detected in an immunocytochemical section of HepG2 cells. The section was incubated with rabbit anti-ATP5F1A Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis using anti- ATP5F1A antibody. detected in paraffin-embedded section of human colon cancer tissue. The tissue section were stained using the Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue).FC
Flow Cytometry analysis of U937 cells using anti-ATP5F1A antibody. Overlay histogram showing U937 cells stained with Anti-ATP5F1A Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP5F1A Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to ATP5F1A |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ATP5F1A recombinant protein (Position: R12-Q520). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:2000-1:10000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP5F1A |
|---|---|
| Gene Synonyms | OMR ORM ATPM MOM2 ATP5A hATP1 ATP5A1 MC5DN4 ATP5AL2 COXPD22 MC5DN4A MC5DN4B HEL-S-123m |
| Gene Full Name | ATP synthase F1 subunit alpha |
| Gene Summary | This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq, Mar 2012] |
| Alternative Names | ATP5A ATP5A1 ATP5AL2 ATPM Cell and organelle markers hATP1 Mitochondria Marker Mitochondrial Marker Mitochondrion Marker MOM2 OMR ORM |
| Molecular Weight(MW) | 60 kDa(Observed band size: 55 kDa) |
| Cellular Localization | Cell membrane,CF(1),Membrane,Mitochondrion,Mitochondrion inner membrane. |
WB
Western blot analysis of ATP5F1A using anti-ATP5F1A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ATP5F1A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ATP5F1A using anti-ATP5F1A antibody. ATP5F1A was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ATP5F1A Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of ATP5F1A using anti-ATP5F1A antibody. ATP5F1A was detected in an immunocytochemical section of HepG2 cells. The section was incubated with rabbit anti-ATP5F1A Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis using anti- ATP5F1A antibody. detected in paraffin-embedded section of human colon cancer tissue. The tissue section were stained using the Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue).
FC
Flow Cytometry analysis of U937 cells using anti-ATP5F1A antibody. Overlay histogram showing U937 cells stained with Anti-ATP5F1A Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP5F1A Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:2000-1:10000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
