WB
Western blot analysis of POMC on rat pituitary tissue lysates with Rabbit anti-POMC antibody at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-POMC antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rabbit anti-POMC antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Application: IF-tissue. Species: Mouse. Site: Pituitary. Sample: Paraffin-embedded section. Antibody concentration: 1:1,000.| Product Name | POMC Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human POMC aa 21-70 / 267. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000 IHC:1:1000 IF-P:1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | POMC |
|---|---|
| Gene Synonyms | LPH MSH NPP POC ACTH CLIP OBAIRH |
| Gene Full Name | proopiomelanocortin |
| Gene Summary | This gene encodes a preproprotein that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the preproprotein and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jan 2016] |
| Alternative Names | ACTH antibody Adrenocorticotropic hormone antibody Adrenocorticotropin antibody alpha melanocyte stimulating hormone antibody Alpha MSH antibody Alpha-MSH antibody beta endorphin antibody Beta LPH antibody beta melanocyte stimulating hormone antibody Beta MSH antibody Beta-LPH antibody Beta-MSH antibody CLIP antibody COLI_HUMAN antibody Corticotropin like intermediary peptide antibody Corticotropin lipotropin antibody Corticotropin-lipotropin antibody Gamma MSH antibody Gamma-LPH antibody Gamma-MSH antibody Lipotropin beta antibody Lipotropin gamma antibody LPH antibody Melanotropin alpha antibody Melanotropin antibody Melanotropin beta antibody Melanotropin gamma antibody met enkephalin antibody Met-enkephalin antibody MSH antibody NPP antibody POC antibody POMC antibody pro ACTH endorphin antibody Proopiomelanocortin antibody Proopiomelanocortin preproprotein antibody |
| Molecular Weight(MW) | 29 kDa(Observed band size: 35 kDa) |
| Cellular Localization | Secreted. |
WB
Western blot analysis of POMC on rat pituitary tissue lysates with Rabbit anti-POMC antibody at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded rat pituitary tissue with Rabbit anti-POMC antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue with Rabbit anti-POMC antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Application: IF-tissue. Species: Mouse. Site: Pituitary. Sample: Paraffin-embedded section. Antibody concentration: 1:1,000.| Application Notes | WB:1:1000 IHC:1:1000 IF-P:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
