WB
Western blot analysis of VAPA using anti-VAPA antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VAPA antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of VAPA using anti-VAPA antibody. VAPA was detected in a paraffin-embedded section of human cervical cancer tissue. The tissue section was incubated with rabbit anti-VAPA Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of VAPA using anti-VAPA antibody. VAPA was detected in a paraffin-embedded section of mouse colon tissue. The tissue section was incubated with rabbit anti-VAPA Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of VAPA using anti-VAPA antibody. VAPA was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-VAPA Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of VAPA using anti-VAPA antibody. VAPA was detected in an immunocytochemical section of PC-3 cells. The section was incubated with rabbit anti-VAPA Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis using anti- VAPA antibody detected in paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue).IF-P
IF analysis using anti- VAPA antibody detected in paraffin-embedded section of rat colon tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue).FC
Flow Cytometry analysis of U87 cells using anti-VAPA antibody. Overlay histogram showing U87 cells stained with Anti-VAPA Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VAPA Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to VAPA |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human VAPA, which shares 94.1% amino acid (aa) sequence identity with mouse and rat VAPA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VAPA |
|---|---|
| Gene Synonyms | VAP-A VAP33 VAMP-A VAP-33 hVAP-33 |
| Gene Full Name | VAMP associated protein A |
| Gene Summary | The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Alternative Names | 33 kDa VAMP associated protein hVAP 33 VAMP A VAMP associated protein A VAP 33 VAP A VAP33 VAPA |
| Molecular Weight(MW) | 28 kDa(Observed band size: 33 kDa) |
| Cellular Localization | Cell junction,Cell membrane,Endoplasmic reticulum,Membrane,Nucleus,Tight junction. |
WB
Western blot analysis of VAPA using anti-VAPA antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-VAPA antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of VAPA using anti-VAPA antibody. VAPA was detected in a paraffin-embedded section of human cervical cancer tissue. The tissue section was incubated with rabbit anti-VAPA Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of VAPA using anti-VAPA antibody. VAPA was detected in a paraffin-embedded section of mouse colon tissue. The tissue section was incubated with rabbit anti-VAPA Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of VAPA using anti-VAPA antibody. VAPA was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-VAPA Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of VAPA using anti-VAPA antibody. VAPA was detected in an immunocytochemical section of PC-3 cells. The section was incubated with rabbit anti-VAPA Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis using anti- VAPA antibody detected in paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue).
IF-P
IF analysis using anti- VAPA antibody detected in paraffin-embedded section of rat colon tissue. The tissue section were stained using the cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue).
FC
Flow Cytometry analysis of U87 cells using anti-VAPA antibody. Overlay histogram showing U87 cells stained with Anti-VAPA Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VAPA Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
