WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LC3B antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Mouse brain was stained with anti-LC3B rabbit antibody.IHC
Rat brain was stained with anti-LC3B rabbit antibody.IHC
Human brain was stained with anti-LC3B rabbit antibody.ICC/IF
Immunofluorescence analysis of Hela cell. 1,mouse Histone H3 Antibody (red) was diluted at 1:200 (4℃ overnight).LC3B Rabbit mAb (green) was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min).IF-P
Immunofluorescence analysis of Mouse-lung tissue. 1,LC3B Antibody (red) was diluted at 1:200 (4℃ overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B| Product Name | LC3B Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 IF-P:1:200-1:1000 |
|
| Species Reactivity | HumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MAP1LC3B |
|---|---|
| Gene Synonyms | LC3B ATG8F MAP1LC3B-a MAP1A/1BLC3 |
| Gene Full Name | microtubule associated protein 1 light chain 3 beta |
| Gene Summary | The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008] |
| Alternative Names | MAP1LC3B |
| Molecular Weight(MW) | 14 kDa,16 kDa |
| Cellular Localization | Cytoplasm. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LC3B antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Mouse brain was stained with anti-LC3B rabbit antibody.
IHC
Rat brain was stained with anti-LC3B rabbit antibody.
IHC
Human brain was stained with anti-LC3B rabbit antibody.
ICC/IF
Immunofluorescence analysis of Hela cell. 1,mouse Histone H3 Antibody (red) was diluted at 1:200 (4℃ overnight).LC3B Rabbit mAb (green) was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min).
IF-P
Immunofluorescence analysis of Mouse-lung tissue. 1,LC3B Antibody (red) was diluted at 1:200 (4℃ overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B| Application Notes | WB:1:1000-1:5000 IHC:1:200-1:1000 ICC/IF:1:200-1:1000 IF-P:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
