WB
Western blot analysis of RBM15 using anti-RBM15 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RBM15 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of RBM15 using anti-RBM15 antibody. RBM15 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-RBM15 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of RBM15 using anti-RBM15 antibody. RBM15 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-RBM15 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of RBM15 using anti-RBM15 antibody. RBM15 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-RBM15 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of RBM15 using anti-RBM15 antibody and anti-Beta Tubulin antibody . RBM15 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-RBM15 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.IF-P
IF analysis of RBM15 using anti-RBM15 antibody. RBM15 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-RBM15 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of 293T cells using anti-RBM15 antibody. Overlay histogram showing 293T cells stained with Anti-RBM15 Antibody (Blue line). To facilitate intrRBM15llular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM15 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to RBM15 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human RBM15 recombinant protein (Position: M1-K967). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RBM15 |
|---|---|
| Gene Synonyms | OTT OTT1 |
| Gene Full Name | RNA binding motif protein 15 |
| Gene Summary | Members of the SPEN (Split-end) family of proteins, including RBM15, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 [PubMed 16129689]).[supplied by OMIM, Feb 2009] |
| Alternative Names | One twenty two protein 1 OTT OTT1 RBM15 RNA binding motif protein 15 SPEN |
| Molecular Weight(MW) | 107 kDa |
| Cellular Localization | Membrane,Nucleus. |
WB
Western blot analysis of RBM15 using anti-RBM15 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RBM15 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of RBM15 using anti-RBM15 antibody. RBM15 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-RBM15 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of RBM15 using anti-RBM15 antibody. RBM15 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-RBM15 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of RBM15 using anti-RBM15 antibody. RBM15 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-RBM15 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of RBM15 using anti-RBM15 antibody and anti-Beta Tubulin antibody . RBM15 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-RBM15 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.
IF-P
IF analysis of RBM15 using anti-RBM15 antibody. RBM15 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-RBM15 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of 293T cells using anti-RBM15 antibody. Overlay histogram showing 293T cells stained with Anti-RBM15 Antibody (Blue line). To facilitate intrRBM15llular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RBM15 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
