WB
Western blot analysis of TRAPA/SSR1 using anti-TRAPA/SSR1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRAPA/SSR1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of TRAPA/SSR1 using anti-TRAPA/SSR1 antibody. TRAPA/SSR1 was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TRAPA/SSR1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of TRAPA/SSR1 using anti-TRAPA/SSR1 antibody. TRAPA/SSR1 was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TRAPA/SSR1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of TRAPA/SSR1 using anti-TRAPA/SSR1 antibody. TRAPA/SSR1 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TRAPA/SSR1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of TRAPA/SSR1 using anti-TRAPA/SSR1 antibody. TRAPA/SSR1 was detected in an immunocytochemical section of K562 cells. The section was incubated with rabbit anti-TRAPA/SSR1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).IP
IP analysis of TRAPA/SSR1 using anti-TRAPA/SSR1 antibody in HepG2 whole cell lysate. Western blot analysis of TRAPA/SSR1 using anti- TRAPA/SSR1 antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- TRAPA/SSR1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.FC
Flow Cytometry analysis of K562 cells using anti-TRAPA/SSR1 antibody. Overlay histogram showing K562 cells stained with Anti-TRAPA/SSR1 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAPA/SSR1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to TRAPA/SSR1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human SSR1 recombinant protein (Position: E53-E286). Human SSR1 shares 98.3% and 90.6% amino acid (aa) sequence identity with mouse and rat SSR1, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 IP:1:250-300 |
|
| Species Reactivity | HumanMonkeyMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SSR1 |
|---|---|
| Gene Synonyms | TRAPA |
| Gene Full Name | signal sequence receptor subunit 1 |
| Gene Summary | The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD glycoprotein. This gene generates several mRNA species as a result of complex alternative polyadenylation. This gene is unusual in that it utilizes arrays of polyA signal sequences that are mostly non-canonical. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014] |
| Alternative Names | SSR alpha SSR1 TRAP alpha TRAPA TRAPA/SSR1 |
| Molecular Weight(MW) | 32 kDa |
| Cellular Localization | Endoplasmic reticulum,Membrane. |
WB
Western blot analysis of TRAPA/SSR1 using anti-TRAPA/SSR1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TRAPA/SSR1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of TRAPA/SSR1 using anti-TRAPA/SSR1 antibody. TRAPA/SSR1 was detected in a paraffin-embedded section of mouse brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TRAPA/SSR1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of TRAPA/SSR1 using anti-TRAPA/SSR1 antibody. TRAPA/SSR1 was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TRAPA/SSR1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of TRAPA/SSR1 using anti-TRAPA/SSR1 antibody. TRAPA/SSR1 was detected in a paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-TRAPA/SSR1 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of TRAPA/SSR1 using anti-TRAPA/SSR1 antibody. TRAPA/SSR1 was detected in an immunocytochemical section of K562 cells. The section was incubated with rabbit anti-TRAPA/SSR1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IP
IP analysis of TRAPA/SSR1 using anti-TRAPA/SSR1 antibody in HepG2 whole cell lysate. Western blot analysis of TRAPA/SSR1 using anti- TRAPA/SSR1 antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- TRAPA/SSR1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
FC
Flow Cytometry analysis of K562 cells using anti-TRAPA/SSR1 antibody. Overlay histogram showing K562 cells stained with Anti-TRAPA/SSR1 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TRAPA/SSR1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 IP:1:250-300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
