WB
Western blot analysis of anti-14-3-3 Sigma/SFN antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 Sigma/SFN antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody. 14-3-3 Sigma/SFN was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of 14-3-3 sigma using anti- 14-3-3 sigma antibody. 14-3-3 sigma was detected in paraffin-embedded section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- cortactin Antibody. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.FC
Flow Cytometry analysis of U2OS cells using anti-14-3-3 Sigma/SFN antibody. Overlay histogram showing U2OS cells stained with Anti-14-3-3 Sigma/SFN Antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 Sigma/SFN Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to 14-3-3 Sigma/SFN |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human 14-3-3 sigma recombinant protein (Position: M1-S248). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SFN |
|---|---|
| Gene Synonyms | YWHAS |
| Gene Full Name | stratifin |
| Gene Summary | This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis. [provided by RefSeq, Aug 2017] |
| Alternative Names | 14 3 3 protein sigma 14-3-3 14-3-3 sigma HME1 SFN stratifin YWHAS |
| Molecular Weight(MW) | 28 kDa |
| Cellular Localization | Cytoplasm,Nucleus,Secreted. |
WB
Western blot analysis of anti-14-3-3 Sigma/SFN antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 Sigma/SFN antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of 14-3-3 Sigma/SFN using anti-14-3-3 Sigma/SFN antibody. 14-3-3 Sigma/SFN was detected in a paraffin-embedded section of human rectal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Sigma/SFN Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of 14-3-3 sigma using anti- 14-3-3 sigma antibody. 14-3-3 sigma was detected in paraffin-embedded section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti- cortactin Antibody. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.
FC
Flow Cytometry analysis of U2OS cells using anti-14-3-3 Sigma/SFN antibody. Overlay histogram showing U2OS cells stained with Anti-14-3-3 Sigma/SFN Antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 Sigma/SFN Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
