WB
Western blot analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 Epsilon/YWHAE antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody. 14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody. 14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody. 14-3-3 Epsilon/YWHAE was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of A549 cells using anti-14-3-3 Epsilon/YWHAE antibody. Overlay histogram showing A549 cells stained with Anti-14-3-3 Epsilon/YWHAE Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to 14-3-3 Epsilon/YWHAE |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human YWHAE recombinant protein (Position: M1-Q255). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | YWHAE |
|---|---|
| Gene Synonyms | MDS HEL2 MDCR KCIP-1 14-3-3E |
| Gene Full Name | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon |
| Gene Summary | This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Two transcript variants, one protein-coding and the other non-protein-coding, have been found for this gene. [provided by RefSeq, Aug 2008] |
| Alternative Names | 14 3 3 protein epsilon 14 3 3E 14-3-3 14-3-3 epsilon KCIP 1 MDCR MDS YWHAE |
| Molecular Weight(MW) | 29 kDa |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Western blot analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 Epsilon/YWHAE antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody. 14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of rat brain tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody. 14-3-3 Epsilon/YWHAE was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of 14-3-3 Epsilon/YWHAE using anti-14-3-3 Epsilon/YWHAE antibody. 14-3-3 Epsilon/YWHAE was detected in an immunocytochemical section of MCF-7 cells. The section was incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of A549 cells using anti-14-3-3 Epsilon/YWHAE antibody. Overlay histogram showing A549 cells stained with Anti-14-3-3 Epsilon/YWHAE Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-14-3-3 Epsilon/YWHAE Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
