WB
Western blot analysis of ABCE1 using anti-ABCE1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCE1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.WB
Western blot analysis of ABCE1 using anti-ABCE1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCE1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a Fluoro 647 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) secondary antibody.IHC
IHC analysis of ABCE1 using anti-ABCE1 antibody. ABCE1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-ABCE1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of ABCE1 using anti-ABCE1 antibody. ABCE1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-ABCE1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of ABCE1 using anti-ABCE1 antibody. ABCE1 was detected in a paraffin-embedded section of human testis tissue. The tissue section was incubated with rabbit anti-ABCE1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of ABCE1 using anti-ABCE1 antibody. ABCE1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-ABCE1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of U251 cells using anti-ABCE1 antibody. Overlay histogram showing U251 cells stained with Anti-ABCE1 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCE1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to ABCE1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ABCE1 recombinant protein (Position: Q141-D599). Human ABCE1 shares 100% amino acid (aa) sequence identity with mouse ABCE1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ABCE1 |
|---|---|
| Gene Synonyms | RLI OABP RLI1 ABC38 RNS4I RNASEL1 RNASELI |
| Gene Full Name | ATP binding cassette subfamily E member 1 |
| Gene Summary | The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the OABP subfamily. Alternatively referred to as the RNase L inhibitor, this protein functions to block the activity of ribonuclease L. Activation of ribonuclease L leads to inhibition of protein synthesis in the 2-5A/RNase L system, the central pathway for viral interferon action. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | ABCE1 RLI RNASEL1 RNASELI RNS4I OK/SW-cl.40 ATP-binding cassette sub-family E member 1 2'-5'-oligoadenylate-binding protein HuHP68 RNase L inhibitor Ribonuclease 4 inhibitor RNS4I |
| Molecular Weight(MW) | 67 kDa |
| Cellular Localization | Cytoplasm,Mitochondrion. |
WB
Western blot analysis of ABCE1 using anti-ABCE1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCE1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
WB
Western blot analysis of ABCE1 using anti-ABCE1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCE1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a Fluoro 647 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) secondary antibody.
IHC
IHC analysis of ABCE1 using anti-ABCE1 antibody. ABCE1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-ABCE1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of ABCE1 using anti-ABCE1 antibody. ABCE1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-ABCE1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of ABCE1 using anti-ABCE1 antibody. ABCE1 was detected in a paraffin-embedded section of human testis tissue. The tissue section was incubated with rabbit anti-ABCE1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of ABCE1 using anti-ABCE1 antibody. ABCE1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-ABCE1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of U251 cells using anti-ABCE1 antibody. Overlay histogram showing U251 cells stained with Anti-ABCE1 Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCE1 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
