WB
Western blot analysis of AATF using anti-AATF antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AATF antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of AATF using anti-AATF antibody. AATF was detected in a paraffin-embedded section of human brain tissue. The tissue section was incubated with rabbit anti-AATF Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of AATF using anti-AATF antibody. AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of AATF using anti-AATF antibody and anti-Beta Tubulin antibody. AATF was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-AATF Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.The section was counterstained with DAPI (Blue).IF-P
IF analysis of AATF using anti-AATF antibody. AATF was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-AATF Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody to AATF |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human AATF recombinant protein (Position: Q5-H552). Human AATF shares 78.5% and 79.9% amino acid (aa) sequence identity with mouse and rat AATF, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:1000-1:8000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AATF |
|---|---|
| Gene Synonyms | DED BFR2 CHE1 CHE-1 |
| Gene Full Name | apoptosis antagonizing transcription factor |
| Gene Summary | The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis. [provided by RefSeq, Jul 2008] |
| Alternative Names | AATF CHE1 DED HSPC277 Protein AATF Apoptosis-antagonizing transcription factor Rb-binding protein Che-1 |
| Molecular Weight(MW) | 63 kDa(Observed band size: 95 kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of AATF using anti-AATF antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AATF antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of AATF using anti-AATF antibody. AATF was detected in a paraffin-embedded section of human brain tissue. The tissue section was incubated with rabbit anti-AATF Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of AATF using anti-AATF antibody. AATF was detected in a paraffin-embedded section of mouse cerebellum tissue. The tissue section was incubated with rabbit anti-AATF Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of AATF using anti-AATF antibody and anti-Beta Tubulin antibody. AATF was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-AATF Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.The section was counterstained with DAPI (Blue).
IF-P
IF analysis of AATF using anti-AATF antibody. AATF was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-AATF Antibody at a dilution of 1:100. Fluoro594-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:1000-1:8000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
