WB
Western blot analysis of ABCG5 using anti-ABCG5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCG5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ABCG5 using anti-ABCG5 antibody. ABCG5 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-ABCG5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of ABCG5 using anti-ABCG5 antibody. ABCG5 was detected in a paraffin-embedded section of mouse stomach tissue. The tissue section was incubated with rabbit anti-ABCG5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of ABCG5 using anti-ABCG5 antibody. ABCG5 was detected in a paraffin-embedded section of rat stomach tissue. The tissue section was incubated with rabbit anti-ABCG5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of ABCG5 using anti-ABCG5 antibody. ABCG5 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-ABCG5 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).IF-P
IF analysis of ABCG5 using anti-ABCG5 antibody. ABCG5 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-ABCG5 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).IP
IP analysis of ABCG5 using anti-ABCG5 antibodyin HepG2 whole cell lysate. Western blot analysis of ABCG5 using anti- ABCG5 antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- ABCG5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Product Name | Rabbit polyclonal antibody to ABCG5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ABCG5 recombinant protein (Position: M1-S88). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCIPWB |
| WB:1:1000-1:5000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 IP:1:250-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ABCG5 |
|---|---|
| Gene Synonyms | STSL STSL2 |
| Gene Full Name | ATP binding cassette subfamily G member 5 |
| Gene Summary | The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions as a half-transporter to limit intestinal absorption and promote biliary excretion of sterols. It is expressed in a tissue-specific manner in the liver, colon, and intestine. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG8. Mutations in this gene may contribute to sterol accumulation and atheroschlerosis, and have been observed in patients with sitosterolemia. [provided by RefSeq, Jul 2008] |
| Alternative Names | ATP-binding cassette sub-family G (WHITE) member 5 |
| Molecular Weight(MW) | 73 kDa |
| Cellular Localization | Cell membrane,Membrane. |
WB
Western blot analysis of ABCG5 using anti-ABCG5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ABCG5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ABCG5 using anti-ABCG5 antibody. ABCG5 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-ABCG5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of ABCG5 using anti-ABCG5 antibody. ABCG5 was detected in a paraffin-embedded section of mouse stomach tissue. The tissue section was incubated with rabbit anti-ABCG5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of ABCG5 using anti-ABCG5 antibody. ABCG5 was detected in a paraffin-embedded section of rat stomach tissue. The tissue section was incubated with rabbit anti-ABCG5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of ABCG5 using anti-ABCG5 antibody. ABCG5 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-ABCG5 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IF-P
IF analysis of ABCG5 using anti-ABCG5 antibody. ABCG5 was detected in a paraffin-embedded section of human colon cancer tissue. The tissue section was incubated with rabbit anti-ABCG5 Antibody at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red) was used as secondary antibody. The section was counterstained with DAPI (Blue).
IP
IP analysis of ABCG5 using anti-ABCG5 antibodyin HepG2 whole cell lysate. Western blot analysis of ABCG5 using anti- ABCG5 antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- ABCG5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 IP:1:250-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
