IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Hippocampus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ZBTB20 Recombinant Rabbit Monoclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Hippocampus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ZBTB20 Recombinant Rabbit Monoclonal Antibody, 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.IF-P
Application: Immunofluorescence (IF-tissue). Species: Mouse. Tissue: Hippocampus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: ZBTB20 Recombinant Rabbit Monoclonal Antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.IF-P
Application: Immunofluorescence (IF-tissue). Species: Rat. Tissue: Hippocampus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: ZBTB20 Recombinant Rabbit Monoclonal Antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.| Product Name | ZBTB20 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse ZBTB20 aa 181-550. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHC |
| IHC:1:200-1:1000 IF-P:1:500 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ZBTB20 |
|---|---|
| Gene Synonyms | HOF DPZF PRIMS ODA-8S ZNF288 |
| Gene Full Name | zinc finger and BTB domain containing 20 |
| Gene Summary | This gene, which was initially designated as dendritic cell-derived BTB/POZ zinc finger (DPZF), belongs to a family of transcription factors with an N-terminal BTB/POZ domain and a C-terminal DNA-bindng zinc finger domain. The BTB/POZ domain is a hydrophobic region of approximately 120 aa which mediates association with other BTB/POZ domain-containing proteins. This gene acts as a transcriptional repressor and plays a role in many processes including neurogenesis, glucose homeostasis, and postnatal growth. Mutations in this gene have been associated with Primrose syndrome as well as the 3q13.31 microdeletion syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2017] |
| Alternative Names | Zinc finger and BTB domain-containing protein 20 BTB/POZ domain zinc finger factor HOF Zinc finger protein 288 Zbtb20 Zfp288 |
| Molecular Weight(MW) | 81 kDa |
| Cellular Localization | Nucleus. |
IHC
Application: Immunohistochemistry (IHC-P). Species: Mouse. Tissue: Hippocampus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ZBTB20 Recombinant Rabbit Monoclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Rat. Tissue: Hippocampus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ZBTB20 Recombinant Rabbit Monoclonal Antibody, 1/200, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
IF-P
Application: Immunofluorescence (IF-tissue). Species: Mouse. Tissue: Hippocampus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: ZBTB20 Recombinant Rabbit Monoclonal Antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.
IF-P
Application: Immunofluorescence (IF-tissue). Species: Rat. Tissue: Hippocampus. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. Primary antibody: ZBTB20 Recombinant Rabbit Monoclonal Antibody, 1/500, overnight at 4°C. Secondary antibody: Goat Anti-Rabbit IgG (488), 1.5 hours at room temperature.| Application Notes | IHC:1:200-1:1000 IF-P:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
