WB
Western blot analysis of DPP4 / CD26 on different lysates with Rabbit anti-DPP4 / CD26 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-DPP4 / CD26 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of Caco-2 cells labeling DPP4 / CD26 with Rabbit anti-DPP4 / CD26 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DPP4 / CD26 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of Caco-2 cells labeling DPP4 / CD26. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
DPP4 / CD26 was immunoprecipitated from 0.2 mg HT-29 cell lysate with DPP4 / CD26 Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using DPP4 / CD26 Recombinant Rabbit Monoclonal Antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HT-29 cell lysate (input) Lane 2: DPP4 / CD26 Recombinant Rabbit Monoclonal Antibody IP in HT-29 cell lysate Lane 3: Rabbit IgG instead of DPP4 / CD26 Recombinant Rabbit Monoclonal Antibody in HT-29 cell lysate Blocking/Dilution buffer: primary antibody dilution. Exposure time: 10 seconds.| Product Name | DPP4 / CD26 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human DPP4 aa 1-766. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:5000 IHC:1:1000 ICC/IF:1:100 FC:1:1000 IP:1-2μg/sample |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DPP4 |
|---|---|
| Gene Synonyms | CD26 ADABP ADCP2 DPPIV TP103 |
| Gene Full Name | dipeptidyl peptidase 4 |
| Gene Summary | The DPP4 gene encodes dipeptidyl peptidase 4, which is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic type II transmembrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Dipeptidyl peptidase 4 is highly involved in glucose and insulin metabolism, as well as in immune regulation. This protein was shown to be a functional receptor for Middle East respiratory syndrome coronavirus (MERS-CoV), and protein modeling suggests that it may play a similar role with SARS-CoV-2, the virus responsible for COVID-19. [provided by RefSeq, Apr 2020] |
| Alternative Names | CD26 antigen antibody ADA-binding protein antibody ADABP antibody ADCP 2 antibody ADCP-2 antibody ADCP2 antibody Adenosine deaminase complexing protein 2 antibody CD 26 antibody CD26 antibody CD26 antigen 3 antibody Dipeptidyl peptidase 4 antibody Dipeptidyl peptidase 4 soluble form antibody Dipeptidyl peptidase IV antibody Dipeptidyl peptidase IV membrane form antibody Dipeptidyl peptidase IV soluble form antibody Dipeptidyl peptidase intestinal antibody Dipeptidylpeptidase 4 antibody Dipeptidylpeptidase IV (CD26 adenosine deaminase complexing protein 2) antibody Dipeptidylpeptidase IV antibody DPP 4 antibody DPP IV antibody DPP IV estoenzyme antibody DPP4 antibody DPP4_HUMAN antibody DPPIV antibody Intestinal dipeptidyl peptidase antibody T cell activation antigen CD26 antibody T-cell activation antigen CD26 antibody TP 103 antibody TP103 antibody |
| Molecular Weight(MW) | 88 kDa(Observed band size:120 kDa) |
| Cellular Localization | Cell membrane, Apical cell membrane, Cell projection, invadopodium membrane, Cell projection, lamellipodium membraneCell junction, Membrane raft. |
WB
Western blot analysis of DPP4 / CD26 on different lysates with Rabbit anti-DPP4 / CD26 antibody at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-DPP4 / CD26 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of Caco-2 cells labeling DPP4 / CD26 with Rabbit anti-DPP4 / CD26 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DPP4 / CD26 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of Caco-2 cells labeling DPP4 / CD26. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
DPP4 / CD26 was immunoprecipitated from 0.2 mg HT-29 cell lysate with DPP4 / CD26 Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using DPP4 / CD26 Recombinant Rabbit Monoclonal Antibody at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HT-29 cell lysate (input) Lane 2: DPP4 / CD26 Recombinant Rabbit Monoclonal Antibody IP in HT-29 cell lysate Lane 3: Rabbit IgG instead of DPP4 / CD26 Recombinant Rabbit Monoclonal Antibody in HT-29 cell lysate Blocking/Dilution buffer: primary antibody dilution. Exposure time: 10 seconds.| Application Notes | WB:1:5000 IHC:1:1000 ICC/IF:1:100 FC:1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
