WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-AMPKγ1 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human kidney was stained with anti-AMPKγ1 Rabbit antibody.| Product Name | AMPKγ1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PRKAG1 |
|---|---|
| Gene Synonyms | AMPKG |
| Gene Full Name | protein kinase AMP-activated non-catalytic subunit gamma 1 |
| Gene Summary | The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| Alternative Names | PRKAG1 5'-AMP-activated protein kinase subunit gamma-1 AMPK gamma1 AMPK subunit gamma-1 AMPKg |
| Molecular Weight(MW) | 38 kDa |
| Cellular Localization | nucleoplasm ,cytosol ,membrane ,nucleotide-activated protein kinase complex ,extracellular exosome |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-AMPKγ1 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human kidney was stained with anti-AMPKγ1 Rabbit antibody.| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
