WB
Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4℃ over night.
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4℃ overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).