WB
Western blot analysis of OSMR on different lysates with Rabbit anti-OSMR antibody at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane.
Exposure time: 3 minutes.
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.