WB
Western blot analysis of MEF2B on different lysates with Rabbit anti-MEF2B antibody at 1/2,500 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 25 seconds; HeLa is a negative control. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: MEF2B Recombinant Rabbit Monoclonal Antibody, 1/2,500 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Colon. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: MEF2B Recombinant Rabbit Monoclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Human. Sample: Daudi (Human Burkitt's lymphoma cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: MEF2B Recombinant Rabbit Monoclonal Antibody, 1/25, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).FC
Application: Flow Cytometry (Intra). Species: Human. Sample: Daudi (Human Burkitt's lymphoma cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 90% Methanol, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: MEF2B Recombinant Rabbit Monoclonal Antibody (1/500, Red) compared with Rabbit IgG Isotype Control (Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (488), 15 minutes at room temperature.| Product Name | MEF2B Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:2500 IHC:1:1000 ICC/IF:1:25 FC:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MEF2B |
|---|---|
| Gene Synonyms | RSRFR2 |
| Gene Full Name | myocyte enhancer factor 2B |
| Gene Summary | The product of this gene is a member of the MADS/MEF2 family of DNA binding proteins. The protein is thought to regulate gene expression, including expression of the smooth muscle myosin heavy chain gene. This region undergoes considerable alternative splicing, with transcripts supporting two non-overlapping loci (GeneID 729991 and 100271849) as well as numerous read-through transcripts that span both loci (annotated as GeneID 4207). Several isoforms of this protein are expressed from either this locus or from some of the read-through transcripts annotated on GeneID 4207. [provided by RefSeq, Jan 2014] |
| Alternative Names | MADS box transcription enhancer factor 2 antibody MDS1 EVI1 antibody Mef2b antibody MEF2B_HUMAN antibody Myocyte enhancer factor 2B antibody Myocyte specific enhancer factor 2B antibody Myocyte-specific enhancer factor 2B antibody PRDM3 antibody RSRFR2 antibody Serum response factor like protein 2 antibody Serum response factor-like protein 2 antibody XMEF2 antibody |
| Molecular Weight(MW) | 38 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of MEF2B on different lysates with Rabbit anti-MEF2B antibody at 1/2,500 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 25 seconds; HeLa is a negative control. Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: MEF2B Recombinant Rabbit Monoclonal Antibody, 1/2,500 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Application: Immunohistochemistry (IHC-P). Species: Human. Tissue: Colon. Sample: Paraffin-embedded section. Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. Wash buffer: 1× TBST. Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: MEF2B Recombinant Rabbit Monoclonal Antibody, 1/1,000, 1 hour at room temperature. Secondary antibody: 20 minutes at room temperature.
ICC/IF
Application: Immunocytochemistry (IF-cell). Species: Human. Sample: Daudi (Human Burkitt's lymphoma cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: MEF2B Recombinant Rabbit Monoclonal Antibody, 1/25, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (488), 45 minutes at room temperature. Counterstain: Beta tubulin (Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).
FC
Application: Flow Cytometry (Intra). Species: Human. Sample: Daudi (Human Burkitt's lymphoma cell). Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 90% Methanol, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: MEF2B Recombinant Rabbit Monoclonal Antibody (1/500, Red) compared with Rabbit IgG Isotype Control (Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (488), 15 minutes at room temperature.| Application Notes | WB:1:2500 IHC:1:1000 ICC/IF:1:25 FC:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
