WB
Western blot analysis of SPOPL using anti-SPOPL antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SPOPL antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of SPOPL using anti-SPOPL antibody. SPOPL was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-SPOPL Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of SPOPL using anti-SPOPL antibody. SPOPL was detected in a paraffin-embedded section of mouse bladder tissue. The tissue section was incubated with rabbit anti-SPOPL Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of SPOPL using anti-SPOPL antibody and anti-Beta Tubulin antibody. SPOPL was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-SPOPL Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.FC
Flow Cytometry analysis of PC-3 cells using anti-SPOPL antibody. Overlay histogram showing PC-3 cells stained with Anti-SPOPL Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPOPL Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.IP
IP analysis of SPOPL using anti-SPOPL antibody in MCF-7 whole cell lysate. Western blot analysis of SPOPL using anti- SPOPL antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- SPOPL antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Light Chain). The signal is developed using ECL Plus Western Blotting Substrate.| Product Name | Rabbit polyclonal antibody to SPOPL |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human SPOPL recombinant protein (Position: H173-Q361). Human SPOPL shares 95.8% amino acid (aa) sequence identity with mouse SPOPL. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 IP:1:250-1:300 |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPOPL |
|---|---|
| Gene Synonyms | BTBD33 |
| Gene Full Name | speckle type BTB/POZ protein like |
| Gene Summary | Enables identical protein binding activity. Involved in negative regulation of protein ubiquitination and proteasome-mediated ubiquitin-dependent protein catabolic process. Part of Cul3-RING ubiquitin ligase complex. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | HIB homolog 2 Roadkill homolog 2 speckle type POZ protein like SPOPL |
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of SPOPL using anti-SPOPL antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-SPOPL antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of SPOPL using anti-SPOPL antibody. SPOPL was detected in a paraffin-embedded section of human thyroid cancer tissue. The tissue section was incubated with rabbit anti-SPOPL Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of SPOPL using anti-SPOPL antibody. SPOPL was detected in a paraffin-embedded section of mouse bladder tissue. The tissue section was incubated with rabbit anti-SPOPL Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of SPOPL using anti-SPOPL antibody and anti-Beta Tubulin antibody. SPOPL was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-SPOPL Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.
FC
Flow Cytometry analysis of PC-3 cells using anti-SPOPL antibody. Overlay histogram showing PC-3 cells stained with Anti-SPOPL Antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SPOPL Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IP
IP analysis of SPOPL using anti-SPOPL antibody in MCF-7 whole cell lysate. Western blot analysis of SPOPL using anti- SPOPL antibody. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- SPOPL antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a mouse anti-rabbit IgG-HRP secondary antibody (Light Chain). The signal is developed using ECL Plus Western Blotting Substrate.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 IP:1:250-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
