IHC
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-SCN1A Polyclonal Antibody, Unconjugated 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCN1A) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.| Product Name | SCN1A | Nav1.1 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | KLH conjugated synthetic peptide derived from human SCN1A : 601-700/2009. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:100-1:500 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SCN1A |
|---|---|
| Gene Synonyms | DEE6 DRVT FEB3 FHM3 NAC1 SCN1 SMEI DEE6A DEE6B EIEE6 FEB3A HBSCI GEFSP2 Nav1.1 |
| Gene Full Name | sodium voltage-gated channel alpha subunit 1 |
| Gene Summary | Voltage-dependent sodium channels are heteromeric complexes that regulate sodium exchange between intracellular and extracellular spaces and are essential for the generation and propagation of action potentials in muscle cells and neurons. Each sodium channel is composed of a large pore-forming, glycosylated alpha subunit and two smaller beta subunits. This gene encodes a sodium channel alpha subunit, which has four homologous domains, each of which contains six transmembrane regions. Allelic variants of this gene are associated with generalized epilepsy with febrile seizures and epileptic encephalopathy. Alternative splicing results in multiple transcript variants. The RefSeq Project has decided to create four representative RefSeq records. Three of the transcript variants are supported by experimental evidence and the fourth contains alternate 5' untranslated exons, the exact combination of which have not been experimentally confirmed for the full-length transcript. [provided by RefSeq, Oct 2015] |
| Alternative Names | DEE6 DEE6A DEE6B DRVT EIEE6 FEB3 FEB3A FHM3 GEFSP2 HBSCI NAC1 Nav1.1 SCN1 SMEI B230332M13 SCN1A_HUMAN SCN1A Sodium channel protein brain I subunit alpha Sodium channel protein type I subunit alpha Voltage-gated sodium channel subunit alpha Nav1.1 SCN1A_RAT sodium voltage-gated channel alpha subunit 1 febrile convulsions 3 sodium channel voltage-gated type I alpha subunit sodium channel voltage gated type I alpha subunit |
| Molecular Weight(MW) | 229 kDa |
| Cellular Localization | Cell membrane,Membrane. |
IHC
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-SCN1A Polyclonal Antibody, Unconjugated 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SCN1A) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.| Application Notes | IHC:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
