WB
Western blot analysis of NSD3 using anti-NSD3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NSD3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Product Name | Rabbit polyclonal antibody to NSD3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human NSD3 recombinant protein (Position: Q46-K1425). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NSD3 |
|---|---|
| Gene Synonyms | KMT3F KMT3G WHISTLE WHSC1L1 pp14328 |
| Gene Full Name | nuclear receptor binding SET domain protein 3 |
| Gene Summary | This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described. [provided by RefSeq, May 2015] |
| Alternative Names | NSD3 Protein whistle WHSC1 like protein 1 WHSC1L1 |
| Molecular Weight(MW) | 162 kDa(Observed band size: 90,200 kDa) |
| Cellular Localization | Chromosome,Nucleus. |
WB
Western blot analysis of NSD3 using anti-NSD3 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NSD3 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
