WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SPARC antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human testis was stained with anti-SPARC Rabbit antibody.| Product Name | SPARC Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000-1:5000 IHC:1:200-1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | SPARC |
|---|---|
| Gene Synonyms | ON ONT OI17 BM-40 |
| Gene Full Name | secreted protein acidic and cysteine rich |
| Gene Summary | This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015] |
| Alternative Names | SPARC ON SPARC Basement-membrane protein 40 BM-40 Osteonectin ON Secreted protein acidic and rich in cysteine |
| Molecular Weight(MW) | 35 kDa(Observed band size: 39 kDa) |
| Cellular Localization | Secreted , extracellular space , extracellular matrix , basement membrane . In or around the basement membrane. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-SPARC antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human testis was stained with anti-SPARC Rabbit antibody.| Application Notes | WB:1:1000-1:5000 IHC:1:200-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
